Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQ----GRFSPDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEECKKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFK-EVKVWSTFNEIYPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPG-EIGVVHSLETKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPEEDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIYKEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPRIDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPKESAYWYKLVSETKTII 5DT7 Chain:D ((24-465)) -MKFAPNFVFGTATSSYQIEGAHDEGGRTPSIWDTFCDTDGKVFEKHNGDVACDHYHRFEEDIQHIKQLGVDTYRFSIAWPRIFPSK-GQFNPEGMAFYKTLATRLQEEGIKPAVTLYHWDLPMWAHEEGGWVNRDSVDWFLDFARVCFEELDGIVDSWITHNEPWCAGFLSYHLGQHAPGH-TDMNEAVRAVHHMLLSHGKAVEMLKGEFNSATPIGITLNLAPKYAKTDSINDQIAMNNADGYANRWFLDPIFKGQYPVDMMNLFSKYV--HTY-DFIHAGDLATIST---PCDFFGINFYSRNLVEFSAASDFLHK------D-------------AYSDYDKTGMGWDIAPSEFKDLIRRLRAEYTD-LPIYITENGAAFDDQLVDGKIHDQNRIDYVAQHLQAVSDLNDEGMNIAGYYLWSLLDNFEWSFGYDKRFGIIYVDFDTQERIWKDSAHWYANVIQTHKA-

General information: TITO was launched using: RESULT: Template: 5DT7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2548 -37521 -14.73 -86.06 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain D : 0.77 3D Compatibility (PKB) : -14.73 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.582

(partial model without unconserved sides chains): PDB file : Tito_5DT7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DT7-query.scw PDB file : Tito_Scwrl_5DT7.pdb: