Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILL----------IVFLFTKVSYLFAPIWQFLAIV--GLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAWGSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSAGDMIQDISKS-----------------TVQSLGSFVGAVATIL--VALLTMPFILFYLLKDGKQLAPYFVKFLPTRMQKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLIPYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTILLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE--- 1PV6 Chain:A ((1-417)) MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDTGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYNILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALGASIVGIMFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATS-ALEVVILKTLHMFEVPFLLVGCFKYITSQFEVRFSATIYLVCFCFF----KQLAMIFMSVLAGNMYESIG--FQGAYLVLGLVALGFTLISVFTLSGPGPLSLLRRQVNEVA

General information: TITO was launched using: RESULT: Template: 1PV6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1742 -218287 -125.31 -572.93 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -125.31 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.293

(partial model without unconserved sides chains): PDB file : Tito_1PV6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PV6-query.scw PDB file : Tito_Scwrl_1PV6.pdb: