Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHENSDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNKNFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK 3N3B Chain:D ((60-147)) ---------------------------------------------------EPYILIVPSYGGGGTA-----GAVPRQVIR-FLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCGVPWLYRFELMGTQSDIENVRKGVTE---

General information: TITO was launched using: RESULT: Template: 3N3B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 305 -50096 -164.25 -569.27 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain D : 0.74 3D Compatibility (PKB) : -164.25 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_3N3B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N3B-query.scw PDB file : Tito_Scwrl_3N3B.pdb: