Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTMPKRQRSDEKAAHHVQLSVESGSRFDAYIRGWMQGTVEQPLLRPSLLPPLTPLPTLAAPSKSGAAQTPATASAATTVGDLSRLCEWTPPSAVMPASRTAVVYDTSMLDHVSPDAGDYERPARLQSTLDHLTVIGLLPCCQRIPARTAKKHELRRVHCRELIDTVDQLDFFMGIQ---EGRGSVIGQDLFASEHTSRAARMAAGCVIEAVKAVLSGAATNAFAIVRPP-GHHAGPANAAGYCLYNNVAVAARAAQAELAAAQAEHNPAGDAQQPRILILDWDVHHCDGTESIFYDDPSVLVISLHQYGNGHGHVLRKVSSAAAKNPAEISTPSKEAITADDLAALLSGGAIEPPPVSAPEASRPEAASDPPQGSTEGRRVRAAVDYNSLAAQIEEEGDAEIAALFGVDLNAASTSSSNSSSASTSSSVSADSTSVARNGTRPVHYAGDTVGVSFDETPRQRATGAEEEELFYPGTGHLNRVGGDATPAAQGRNINIPWPTHGMGDLEYLQLLHEVVLPAAREFRPELVLISCGFDSASGDLLGSMCLTPSGYYIMTRLMAQNFPKLVVALEGGYNVRNVALCSEAVMRALLESSGCPGDRLPK-----------------SRMLWCQASSLVADIKRMHAPYWSCFSPNM 5ICN Chain:B ((8-375)) ------------------------------------------------------------------------------------------------RRKVCYYYDGDVGNYYYG-QGHPMKPHRIRMTHNLLLNYGLYRKMEIYRPHKANAEEMTKYHSDDYIKFLRSIRPDNMSEYSKQMQRFNVGEDCPVFDGLFEFCQLSTGGSVASAVKLNKQQ--TDIAVNWAGGLHHAKKSEASGFCYVNDIVLAILELLK---------------YHQRVLYIDIDIHHGDGVEEAFYTTDRVMTVSFHKYG-----------------------------------------------------------------------------------------------------------------------------------------------------------------EYFPGTGDLRDIGAG---KGKYYAVNYPLRDG-IDDESYEAIFKPVMSKVMEMFQPSAVVLQCGSDSLSGDRLGCFNLTIKGHAKCVEFVKSFNLPMLMLGGGGYTIRNVARCWTYETAVALDTEIPN--ELPYNDYFEYFGPDFKLHISPSNMTNQNTNEYLEKIKQRLFENLRMLPH--

General information: TITO was launched using: RESULT: Template: 5ICN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2151 -83033 -38.60 -239.29 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -38.60 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.448

(partial model without unconserved sides chains): PDB file : Tito_5ICN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ICN-query.scw PDB file : Tito_Scwrl_5ICN.pdb: