Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQLKPQEVVRLGDIQMANHLPFVLFGGMNVLESKDLAFEIAETYIDICKRLDIPYVFKASFDKANRSSLHSFRGPGLEKGIEWLGDIKKHFNVPIITDVHEPYQAAPVAEVADIIQLPAFLSRQTDLVEAMAKTQAIINIKKAQFLAPHEMRHILHKCLEV-------------GNDKLILCERGSAFGYNNLVVDMLGFDIMKEMNVPVFFDVTHALQTPGGRSDSAGGRRAQITTLARAGMATGLAGLFLESHPDPDKAKCDGPSALRLSQLEPFLAQLKELDTLVKGFKKLDTH 4Z1D Chain:B ((10-275)) ---------------------KSVLIAGPCVIESLENLRSIATKLQPLANNERLDFYFKASFDKANRTSLESYRGPGLEKGLEMLQTIKEEFGYKILTDVHESYQASVAAKVADILQIPAFLCRQTDLIVEVSQTNAIVNIKKGQFMNPKDMQYSVLKALKTRDKSIQSPTYETALKNGVWLCERGSSFGYGNLVVDMRSLKIMRE-FAPVIFDATHSVQMP-----------SFAPILARAAAAVGIDGLFAETHVDPKNALSDGANMLKPDELEQLVTDMLKIQNL----------

General information: TITO was launched using: RESULT: Template: 4Z1D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1400 -89534 -63.95 -369.97 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -63.95 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.496

(partial model without unconserved sides chains): PDB file : Tito_4Z1D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Z1D-query.scw PDB file : Tito_Scwrl_4Z1D.pdb: