Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALVKTKLAAAPQPSRTPTPGSSPKEWTPPTHTHESPEHLSTDEPDVLVPA----HKYVYDVKTCAWKGVDTMLRVLHPNRGLSQGTMRVCFALEELD----ETGFSSRMVAKMFRHNISKV---------VESDYFNEGEAQCICGIFAEKFNRVQVPAGFQRHIVSFLQCETVRIKLSEVPEAYQHKRSGFFSYRTTDSADILFTMEPRLAGNFTKYTNNFGDVYDGFERRQSLEEEKKRHRVLMAVEAFSHFTLVESGGSMLVCDLQGVNDFLTDPQIHTEDG-----KGLGMGNMGQDGIRKWMEAHVCNEVCRAIMLEPLSKGLRNFTRTAENESRVSYYQILRAKLRSQTPVRPEDIIPLSKPLSLMSDDERLEYAMRLSALLSE 1IAH Chain:B ((28-277)) ------------------------------------------RGEPVTVYRLEESSPSILNNSMSSWSQLGLCAKIEFLSKEEMGGGLRRAVKVLCTWSEHDILKSGHLYIIKSFLPEVINTWSSIYKEDTVLHLCLREIQQQRAAQKLTFAFNQMKPK-SIP-YSPRFLEVFLLYCH----------------------SAGQWFAVEECMTGEFRKYNNNNGDEIIPT------------NTLEEIMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKAEEKRSCDMVFGPANLGEDAIKNFRAKHHCNSCCRKLKLPDLKRN---------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1IAH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1127 -13567 -12.04 -59.50 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -12.04 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.338

(partial model without unconserved sides chains): PDB file : Tito_1IAH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1IAH-query.scw PDB file : Tito_Scwrl_1IAH.pdb: