Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------------------MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVI---YQLSHQELLATALANIIGILFAFITNDTIVFKQKK--KNRLIRLV-----KFSLARLSTFLLDL------FLTFLFVTQFPHII---GQFVTENIDKINAIETIIAQ-----IFIIIINYILSKVYIFRK------------ 5TCX Chain:A ((6-232)) STKSIKYLLFVFNFVFWLAGGVILGVALWLRHP--NTFYVGIYILIAVGAVMMFVGFLGYGAIQQLLGTFFTCLVILFACEVAAGIWGFVNKDQIAKDVKQFYDQALQQAVVDDDANNAKAVVKTFHETLDCCGSSTLTALTTSVLKNNLCPSGSNIISNLFKEDCHQKIDDLFSGKLYLIGIAAIVVAVIMIFEMILSMVLSSGIRN

General information: TITO was launched using: RESULT: Template: 5TCX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 360 -48430 -134.53 -343.48 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -134.53 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.176

(partial model without unconserved sides chains): PDB file : Tito_5TCX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TCX-query.scw PDB file : Tito_Scwrl_5TCX.pdb: