Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSITLLQPDDWHAHLRDGLALKRTVPDLAKQFARAICMPNLVPPVKTVEEALAYRERILAHVPEGNNFDPRMVLYFTDHTSPDEVRKIKESEHVNAIKLYPAGATTNSNNGVSDIRKVYAVIEQLEEHQVPLLLHGEVTHNHVDIFDREKRFLDEVLSPLLKQFPKLKVVLEHITTSDAAHFVLEQDRNVAATITPQHLLFNRNDMLVGGIKPYFYCLPILKRQTHQTTLLEVATSGNPKFFLGTDSAPHAQNAKENACGCAGCYSAPNAIELYAQAFDQVGKLERLEGFASHFGADFYGLPRNTSTITLVKEDNLVPESFDYLDNQKIIPLHAGKTLQWRKV 5V0G Chain:B ((30-368)) -QTLKIRRPDDWHIHLRDDEMLSTVLPYTSEVFARAIVMPNLAQPITTVASAIAYRERILAAVPAGHKFTPLMTCYLTNSLDAKELTTGFE-QGVFTAALYPANATTNSTHGVSDIPAIYPLFEQMQKIGMPLLIHGEVTDAAVDIFDREARFIDQILEPIRQKFPELKIVFEHITTKDAADYVLAGNRFLGATVTPQHLMFNRNHMLVGGIRPHLFCLPILKRSTHQQALRAAVASGSDRFFLGTDSAPHAKHRKESS--GAGVFNAPAALPAYASVFEELNALQHLEAFCALNGPRFYGLPVNDDVVELVRTPFLQPEEIP-LGNESVIPFLAGQTLNW---

General information: TITO was launched using: RESULT: Template: 5V0G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1924 -197471 -102.64 -587.71 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : -102.64 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.494

(partial model without unconserved sides chains): PDB file : Tito_5V0G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5V0G-query.scw PDB file : Tito_Scwrl_5V0G.pdb: