Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKGPDMQAIILDTETHTLNGLPIEIAYAPIEINAGKLTLDKSKLFDQLYQVGTPISYAAMAVHHILESDLENQPHYKTFK-LPDTTTYIIGHNIDYDIAAIARCGVDVSHIKPICTLALTRKTWPDAEAHNISALI--YMISQGSSKARELLKGAHRADADIILTANILMHIVYHLNIHDIEELYRVSEEARIPTTINFGKHKGTAIAELPKDYIQWLLRQDELDVYLRKALESAF 4FZY Chain:A ((1-155)) -------MLRIIDTETCGLQGGIVEIASVDVIDG------KIVNPMSHLVRPDRPISPQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEM-----PGEWICTMKLARRLWPGI-KYSNMALYKTRKLNVQTP----PGLHHHRALYDCYITAALLIDIMNTSG-----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4FZY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 771 -63273 -82.07 -416.27 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -82.07 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.294

(partial model without unconserved sides chains): PDB file : Tito_4FZY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FZY-query.scw PDB file : Tito_Scwrl_4FZY.pdb: