Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNLQNPKKFKMMIDQAHDVAINVLRPISRKYD-KAEHAYPKELDMLASL-IDGMNE----GGEGINAGAAGAGKRGESDNEGVRNGTNMSTALGIIEMCYGDTGLLLSMPRQGLGNSAIAAVANDEQLERF-----KGTW-AAMAITEPGCGSDSAAIRTTATKDGNDYILNGEKIFVTSGERADSVVVWATLDRKLGRAAIKSFVVPKGTPGMKVERLEHKLGIKASDTAVISFTDCRVPAENLLGNPEIDVAKGFAGVMETFDNTRPLVAAMAIGCAKASLERIKEIFKDQLDSNYSTPYLQTSNLAAQIYRMEAEWEAARLLMLKATWMADNKKPNSKEASIAKAKAGRVGNEITLKCVELAASVGYKEDELLEKWARDSKILDIFEGTQQIQQLIIARRELGKSSSELK 2DVL Chain:A ((8-368)) ---------RLVLDAVRRVAREVLYPLAPEYDRKAEYPWP-QLKALAELGLLGMTTPEEWGGVGLDS-------------------VTWALALEELAAADPSVAVIVSVT-SGLPQYMLLRFGSEAQKRRYLVPLARGEWIGAFCLTEPQAGSDAKSLRAEARRVKGGFVLNGVKSWITSAGHAHLYVVMARTEK-----GISAFLVEKGTPGLSFGRPEEKMGLHAAHTAEVRLEEVFVPEENLLG----EEGRGLAYALAGLDSGRVGVAAQAVGIARGAFEIAKAYAEER--EQFGKKLKEHQAIAFKIADMHVKIAAARALVLEAARKKDRGERFTLEASAAKLFASAAAVEVTREAVQVLGGYGYHRDYRVERYYRDAKVTEIYEGTSEIQRLVIAR-----------

General information: TITO was launched using: RESULT: Template: 2DVL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2058 47834 23.24 137.06 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : 23.24 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.514

(partial model without unconserved sides chains): PDB file : Tito_2DVL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2DVL-query.scw PDB file : Tito_Scwrl_2DVL.pdb: