Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSIVYAFVGGLLLGIATVGYLYINGRIAGISGLLAQFISPSRDIFK----SSAFWFIAGLVITPFIYGYFYQPEIEI-------KANSFVLILAGLLVGFGTRLGSGCTSGHGICGMSRLSKRSIIASAIFMFAGMLTVYIIRHVLG-------- 2BL2 Chain:A ((1-156)) MMDYLITQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAALTTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGSDMSVVQGLNFLGASLPIAFTGLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA

General information: TITO was launched using: RESULT: Template: 2BL2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 568 -64213 -113.05 -468.70 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -113.05 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.169

(partial model without unconserved sides chains): PDB file : Tito_2BL2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BL2-query.scw PDB file : Tito_Scwrl_2BL2.pdb: