Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNALTQELVELLTLEKLEENIYRGISRNLVGKRVFGGQVLGQALRAASYTT-DRPAHSLHAYFLYGGDINAPIIYEVDRLRDGKSFVSRQVRAIQHGRVIFSAMVSFANPEEG-LNYQHPEPDYPAPEALKSESELKEGILNFVPENVRASFMRERHVEIRPIDPVNPFQPQPEAPFNAHYIRTHDRIPKQLEDISLHQAIVAFYSDFTLMTTALRPHGLSYISPSL-QCASIDHAIYFHRPLRADEWMLYDMEATVSAASRGLNFGRMW-QNGQLVCSTVQEGLMRLREIETQ 4R9Z Chain:A ((4-272)) ---SLGDVLATLELERVGQWRFVGQQLPAPANHILGGHISAQALLAASRTAAGREPHSVHTYFLRPGDSRQPVDFEVVDLQEGRTFSARRVTARQDDKILMEAMSSFKV----QVVYQPIMPEAPSPESL--------------------AS--LRWFERRTIETE-----TVPPARVPMWWRPDGRVPD---DPVLTASLVAYMSAVTLTEPAFAAR------GGVGASAQRDHSVWFHGRAVLSDWLFYDRSSPSSAGSLALASGTMFNRTGELVCTVKQEMYFPP------

General information: TITO was launched using: RESULT: Template: 4R9Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1411 -24915 -17.66 -103.38 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : -17.66 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.361

(partial model without unconserved sides chains): PDB file : Tito_4R9Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R9Z-query.scw PDB file : Tito_Scwrl_4R9Z.pdb: