Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMTEQKSALSLRYFLNLEESQDGFGLVTFGKKQFTRFLTPVISIAIIIWGLYLGFSGVGRYYVALGVFFLVMQGLMRYWLLPMMFKRQFVRYQFGKSEQGIDLYQDYFEFYAAGRKQAVQYSEVQTFAKG-------KLTYMLELKNKTVI-----IVPKRAFAQASDQTIFENTFKK------------------------------- 5TCX Chain:A ((6-232)) STKSIKYLLFVFNFVFWLAGGVILGVALWLRHPNTFYVGIYILIAVGAVMMFVGFLGYGAIQQLLGTFFTCLVILFACEVAAGIWGFV---NKDQIAKDVKQFYDQALQQAVVDDDANNAKAVVKTFHETLDCCGSSTLTALTTSVLKNNLCPSGSNIISNLFKEDCHQKIDDLFSGKLYLIGIAAIVVAVIMIFEMILSMVLSSGIRN

General information: TITO was launched using: RESULT: Template: 5TCX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 498 -35875 -72.04 -220.09 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -72.04 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.150

(partial model without unconserved sides chains): PDB file : Tito_5TCX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TCX-query.scw PDB file : Tito_Scwrl_5TCX.pdb: