Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTRHFSQLPKAALAYPKVVQGLIFKKPKGPKILPQVEYVVDTLEIDQNHLKAYNEVCGFQNNGFVPAIYLAVLSQSLQMHMMTAEAFPFPILGLVHIRNQIKQTRPIGVTEKLTLSCKLGELKPHDKGVQFDFITTAKVGNEVVMEGLTTYLSRQKVEKRVGEKAKEEQAPAYVPKAEWNILENTGRRYAKVSGDFNLIHIHAITAKAFGFKQAIAHGMWSKAKALANLELPNAYEADVWFKLPMFLPSKVEFLTANADKKTDFLIRNAKSQKPHVAGTVKAL 4V12 Chain:A ((216-265)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------EELPVHTARLSRGDLVN----YAGVAGDANPLHWDENIAKLAGQPDVIAHGMLT--------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4V12.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 120 -16726 -139.38 -334.52 target 2D structure prediction score : 0.32 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -139.38 2D Compatibility (Sec. Struct. Predict.) : 0.32 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.401

(partial model without unconserved sides chains): PDB file : Tito_4V12.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4V12-query.scw PDB file : Tito_Scwrl_4V12.pdb: