Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------MDSRMEEAKISLDALVPKNHLVHKIDKILLFDFIYPIVESTYSTIGRPSVDPVIL-----------VKLVFIQYLFRIRSMR-QSIKEAETNLAYRWFLGLSLI----DKIPHFSTFGKNY-----VRHFRETDCLNRYLSLSLN-KQLKLDLFMKKFCIW-----------------------------------IQPILKRMRKNIF-LQEKWYINKPFYTKNN 5F2T Chain:A ((44-290)) GGPEQLRRNLARVVGKPPADVPDDLIRASLASYARYWREAFRLPAMDHGRLGEQLDVIDIDHLWSALDAGRGAVLALPHSGNWDMAGVWLVQNYGPFTTVAERLKPESLYRRFVEYRESLGFEVLPLTGGERPPFEVLAERLTDNRPICLMAERDLTRSGVQVDFFGEATRMPAGPAKLAIETGAALFPVHCWFEGDGWGMRVYPELDTSSGDVTAITQALADRFAANIATYPADWHMLQPQWIADL

General information: TITO was launched using: RESULT: Template: 5F2T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 493 28596 58.00 170.21 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 58.00 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.249

(partial model without unconserved sides chains): PDB file : Tito_5F2T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F2T-query.scw PDB file : Tito_Scwrl_5F2T.pdb: