Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDEEQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKNGKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAK-LGYDMLSNSLRNVVKSSKFLRPQLKIMNSKIQ-HLPSNSFAMALASE--TSTLDGFA-PTTAILDEWHEAKTRKTYNVIKSGMTQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQEKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIAEDWQATEVE--PQ-DITGKPVYIGVDLSKT-DDLTSVSWIVPLDNGELYCDSHSFVATKYGLQDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPYNAN-SLISKAEKA--N--YPMLEVRQGT---------------------ITLNVPTRTFREQVYEGN---------------------VIHNKNTILTHAVNNAILKTDNNG-IQI-NKS---KN--SNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF 4IEE Chain:A ((35-478)) ------------------------------------------------------------------------------------------HRYKVAKGGRGSGKSWAIARLLVEAARRQ-----PVRILCARELQNSISDSVIRLL----EDTIER-EGYSAEFEIQRSMIRHL-GTNAEFMFYGIKNNPTKIKSLEGIDICWVEEAEAVT-KESWDILIPTIRK-PFSEIWVSFNPKNI-LDDTYQRFVVNP--------PDDICLLTVN------------Y-TDNPHFPEV---L--RLEMEECKR-----RNPTLYRHIWLGEPVSASDMAIIKREWLEAATDAHKKLGWKAKGAVVSAHDPSDTGPDAKGYASRHG---SVVKRI-AEG--------------------------------LL--MDINEGADWATSLAIE--DGADHYLWDGDGVGAGLRRQTTEAFSGKKITATMFKGSESPFDEDAPYQANVRTIGDVFRNKRAQFYYALADRLYLTYRAVVHGEYADPDDMLSFDKEAIGEKMLEKLFAELTQIQRKFNNNGKLELMTKVEMKQKLGIPSPNLADALMMCMHCPALVRE-------------------

General information: TITO was launched using: RESULT: Template: 4IEE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1742 55917 32.10 151.13 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 32.10 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.377

(partial model without unconserved sides chains): PDB file : Tito_4IEE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IEE-query.scw PDB file : Tito_Scwrl_4IEE.pdb: