Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------NCIANILNINQAVIATGCIPAGGQRLIRVGSSHSYLIRATTSC---GLGLTTTQRFINGESVASGGRC 2L7X Chain:A ((1-77)) RELKPQTCTICETTPVNAIDAEMHDLNCSYNICPYCASRLTSDGLARHVTQCPKRKEKVEETELYLNLERIPWVVRK

General information: TITO was launched using: RESULT: Template: 2L7X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 258 2855 11.07 43.92 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 11.07 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.016

(partial model without unconserved sides chains): PDB file : Tito_2L7X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2L7X-query.scw PDB file : Tito_Scwrl_2L7X.pdb: