Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence CYGPISMDFAISKLKKAGNQTGLYVLRCSPKDFNKYFLTFAVERENVIEYKHCLITKNENEEYNLSGTKKNFSSLKDLLNCY 4EIH Chain:A ((15-89)) YHGPVSRSAAEYLLS--SLINGSFLVRESESSPGQLSISLRYEG--R-V-YHYRINTTADGKVYVTA-ESRFSTLAELVHHH

General information: TITO was launched using: RESULT: Template: 4EIH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 270 -43868 -162.47 -584.91 target 2D structure prediction score : 0.80 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -162.47 2D Compatibility (Sec. Struct. Predict.) : 0.80 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.637

(partial model without unconserved sides chains): PDB file : Tito_4EIH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EIH-query.scw PDB file : Tito_Scwrl_4EIH.pdb: