Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------MEFFGNKPFTQQPQRAITQANQLLDYK--------SWSEEDRKMFSQLHMREEQVLLAQDYALETARAEDLEQGLERGKVEGRAERKLFTFLDIVRQGLLTSEVASQQLGMSVSEFEALL- 2EO1 Chain:A ((1-102)) GSSGSSGKVVFAKEQPAHREVQAEAGASATLSCEVAQAQTEVTWYKDGKKLSSSSKVRVEAV------------------GCTRRLVVQQAGQA--------EAGEYSCEAGGQQLSFRLQVAGQCFG

General information: TITO was launched using: RESULT: Template: 2EO1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 282 23653 83.88 275.03 target 2D structure prediction score : 0.19 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 83.88 2D Compatibility (Sec. Struct. Predict.) : 0.19 1D Compatibility (Hydrophobicity) : 0.65 QMean score : -0.062

(partial model without unconserved sides chains): PDB file : Tito_2EO1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EO1-query.scw PDB file : Tito_Scwrl_2EO1.pdb: