Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIQIIVNTFIEKDKTGAVVEV--L--YASADQDKVQAKYEELAAQYPENYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE 1SVD Chain:M ((52-91)) --------------MNQYWYMWKLPFFGEQNVDNVLAEIEACRSAYPTHQVKLV-------------------------

General information: TITO was launched using: RESULT: Template: 1SVD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain M - contact count / total energy / energy per contact / energy per residue : 31 -4139 -133.52 -114.97 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain M : 0.61 3D Compatibility (PKB) : -133.52 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.665

(partial model without unconserved sides chains): PDB file : Tito_1SVD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SVD-query.scw PDB file : Tito_Scwrl_1SVD.pdb: