Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGIKMEKIFVIIFFVCLFISSITFLAY------DFVSEEIKKLIIWMNVVFLILIIAMIIYPKLRK------------ 5U1T Chain:B ((1-117)) MDIEIAP------------QRQEPLPYVPEGYSPFQQDDIEKLKTFNSPYKLDLDKVDLLPLEQIEGLDPEELEDLVT

General information: TITO was launched using: RESULT: Template: 5U1T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 11 1784 162.14 37.16 target 2D structure prediction score : 0.21 Monomeric hydrophicity matching model chain B : 0.50 3D Compatibility (PKB) : 162.14 2D Compatibility (Sec. Struct. Predict.) : 0.21 1D Compatibility (Hydrophobicity) : 0.50 QMean score : 0.226

(partial model without unconserved sides chains): PDB file : Tito_5U1T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5U1T-query.scw PDB file : Tito_Scwrl_5U1T.pdb: