Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINFINSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENSSEFVQTRVITEN 1S12 Chain:D ((1-91)) MIKVTVTN------SFFEVTGHAPD-----KTLCASVSLLTQHVANFLKAEKKA--KI--KK-ESGYLKVKFEE-L----ENCEVKVLAAMVRSLKELEQKFPSQIRVEVID--

General information: TITO was launched using: RESULT: Template: 1S12.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 360 -65936 -183.15 -724.57 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain D : 0.75 3D Compatibility (PKB) : -183.15 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.523

(partial model without unconserved sides chains): PDB file : Tito_1S12.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1S12-query.scw PDB file : Tito_Scwrl_1S12.pdb: