Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITFYLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQGDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCILMLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKR--PDQLAKAINDDLGRGVTFISGQGYYSKENLKIIYCIV-GRNEIVKTKEMIH-RIDP--QAFITITEAHEILGEGFTFEKE 3DFE Chain:B ((7-102)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NKLVIVTEKVLLKKVAKIIEEAGATGYTVVDTGG-----SNVKFEVLTENREMAEKIADQVAIKFFTDYAGIIYICEAEVLYG--------

General information: TITO was launched using: RESULT: Template: 3DFE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 315 -541 -1.72 -7.51 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.43 3D Compatibility (PKB) : -1.72 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.43 QMean score : 0.599

(partial model without unconserved sides chains): PDB file : Tito_3DFE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DFE-query.scw PDB file : Tito_Scwrl_3DFE.pdb: