Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPRYEGSSDCHKSREIVSLDITVNYCHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKLKPLTAEDKACNHA-LSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLSAGIINHELGF 3CSY Chain:J ((1-98)) EAIVNAQPKCNPNLHYWTT-----------------------QDEGAAIGLAWIPYFGPAAEGIYTEGLMH--NQDGLICGLRQLANETTQALQLFLRATTELRTFSILNRKAIDFLLQ----------RWGG

General information: TITO was launched using: RESULT: Template: 3CSY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain J - contact count / total energy / energy per contact / energy per residue : 162 2836 17.50 29.23 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain J : 0.64 3D Compatibility (PKB) : 17.50 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.189

(partial model without unconserved sides chains): PDB file : Tito_3CSY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CSY-query.scw PDB file : Tito_Scwrl_3CSY.pdb: