Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLMNMIGKLEPYD-GTIFYRGKDL-ANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG-QKLSRSEQRLRQKQALEQVGL-----VYLDLDKRIFELSGGESQRVALAKIILKNPPFILADEPTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIW-EMADEVFTMDHLK 2OLJ Chain:A ((24-233)) MIDVHQLKKSFGSLEVLKGINVHIREGEVVVVIGPSGSGKSTFLRCLNLLEDFDEGEIIIDGINLKAKDTNLNKVREEVGMVFQRFNLFPHMTVLNNITLAPMKVRKWPREKAEAKAMELLDKVGLKDKAHAYPD------SLSGGQAQRVAIARALAMEPKIMLFDEPTSALDPEMVGEVLSVMKQLANEGMTMVVVTHEMGFAREVGDRVLFMD---

General information: TITO was launched using: RESULT: Template: 2OLJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 993 -57123 -57.53 -284.19 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -57.53 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.513

(partial model without unconserved sides chains): PDB file : Tito_2OLJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OLJ-query.scw PDB file : Tito_Scwrl_2OLJ.pdb: