Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRRTRMSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSGIDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVLACLREPLPASHYMIAVAGKD-VRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAGAQNLLNAFNIVEEVEYCAKIYCLAKNFGEPV-VLPDEEMELMAEKFKTYGQRK 1E4A Chain:P ((1-205)) MERNKLA---RQIIDTCLEMTRLGLNQGTG-NVSV--RYQDGMLITPTGIPYEKLTESHIVFIDGNGKHEEG-KLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVSILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIACEVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKF-------

General information: TITO was launched using: RESULT: Template: 1E4A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 1086 -79164 -72.89 -389.97 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain P : 0.83 3D Compatibility (PKB) : -72.89 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.526

(partial model without unconserved sides chains): PDB file : Tito_1E4A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E4A-query.scw PDB file : Tito_Scwrl_1E4A.pdb: