Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------MIGQQRN---ILATLGIDVWIPRT-QVCQK-NNA-------------HTLWRDQVVEPHESITVPTIDVPAFEQKNTQPQVFEIPKVVEEPPIVVAEVSQPEILVEKPKVIEQE-TITP-FELQAYCLEKCVIFV-DVTALETEEKQLWANIQKAKVGRYSELRWPFPLAA-YQDQRGVGSYIQGFLDAVAAEKKILCLGKCAYIQHANIIHLA-SLKEMLDKP-LLKKRLWQLMQDNNE 2QGN Chain:A ((2-314)) KEKLVAIVGPTAVGKTKTSVMLAKRLNGEVISGDSMQVYRGMDIGTAKITAEEMDGVPHHL--IDIKDPSESFSVADFQDLATPLITEIHERGRLPFLVGGTGLYVNAVIHQFNLGETPSPYNLVMIGLTMERDVLYDRINRRVDQMVEEGLIDEAKKLYDRGIR----DCQSVQ-AIGYKEMYDYLDGNVTLEEAIDTLKRNSRRYAKRQLTWFRNKANVTWFDMTDVDFDKKIMEIHNFIAGKLEEKSK

General information: TITO was launched using: RESULT: Template: 2QGN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 987 26224 26.57 125.47 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 26.57 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.169

(partial model without unconserved sides chains): PDB file : Tito_2QGN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QGN-query.scw PDB file : Tito_Scwrl_2QGN.pdb: