Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKFLPVILSLALITNASYANPFDPKPASGQVEIPNIGSGIGLLDQQKEKFIG-----EKVFRE-VHKQMPVIQDVWLEDQFFQVFSNILSETQLGQPIALVVIKDPQINAFAVPGGLFALNTGLISSARNI--------DEIAGVMAHEIAHVSQRHFSRSEEAFKGQTLLSLAGLLAGVALAAQAGGDAGAAVMLGTQAALLDKQLTYSRNQEREAD-----RIGMQYMYAAGYNPQSMADYFETMHRATSRVSFLPDFWLTHPLTSERMSEARLRANQMPKVKSRIYDVDFEILKWYTMVVAGEATENQLQSLASQKNLAG----LLALSAFYLKQGDYTQAQATLEQAKSSG-KPLVALIQTDIYLGQNKINQAYN-----------SIAPLQMTMPENKAFSYKLAEVLLRQG--KYAQVQTLVQRFINKNARDIQGWQLLQQAANLDKNSPLRAVNVLRYRAEAQYWSGSEEDAIKSMLHAQRLAKGNQAMSARIDSRLKQMQDERRMKI---------- 5CTQ Chain:A ((44-561)) -----NQLEIERLEEQLSINVYDYNCHVDLIRLLRLEGELTKVRMARQKMSEIFPLTEELWLEWLHDEISMAQDGLDREHVYDLFEKAVKDYIC--PNIWLEYGQYSVGGIGQKGGLEKVRSVFERALSSVGLHMTKGLALWEAYREFESAIVEAARLEKVHSLFRRQLAIPLYDMEATFAEYEEWSEDPIPESVIQNYNKALQQLEKYKPYEEALLQAEAPRLAEYQAYIDFEMKIGDPARIQLIFERALVENCLVPDLWIRYSQYLDRQLKVKDLVLSVHNRAIRNCPWTVALWSRYLLAMERHGVDHQVISVTFEKALNAGFIQATDYVEIWQAYLDYLRRRVDFKQDSSKELEELRAAFTRALEYLKQEVEERFNESGDPSCVIMQNWARIEARLCNNMQKARELWDSIMTRGNAKYANMWLEYYNLERAHGDTQHCRKALHRAVQCTSDYPEHVCEVLLTMERTEGSLEDWDIAVQKTETRLARVNEQRMKAAEKEAALVQQEEEKAEQRKRARAEKKAL

General information: TITO was launched using: RESULT: Template: 5CTQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1841 78522 42.65 166.71 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 42.65 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.272

(partial model without unconserved sides chains): PDB file : Tito_5CTQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5CTQ-query.scw PDB file : Tito_Scwrl_5CTQ.pdb: