Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKQLPKISTTSKALAKSLLLAQG-VLDQAKKYSTLPFTQTHIILPRIDEKYYSWTHYGIFFPLLPEPHRYLNIMILIGTPGALAFDHDDIITGNPRKTATFFSSTAALEQALLKAYII--PEDTKINKDGTLIELGQEISIQGKFPHIHINGHYDGFDFDFDIDITSHVSWFIKTPIYDHFSLLAKFKGFLNYQAKHIETQGLCTYEYARAVGPHSITNKLIPDAYKLPLDFFTYQIINLNEATQLLLTKADIAGQTAAYTLHIRHLDQPAEIYTDVSFDIISHQVDDFVSPSGQKMRLPKYFSWIARNDAKQIILNIQAEIDCPFRYGHGRGYASSYIFTGHYFGNEVQGRGYIEYVDIENPQAFEDE------------------------ 5CZL Chain:A ((23-337)) LPRAWA--DTAWESYKSRFMMADGRIVDTGNGSVSHTEGQGFAMLLAVAKND---------RPAFDKLWQWTDKTLRNKDNGLFYWRYNPVAPDPIADKNDATDGDTLIAWALLRAQQQWGDKSYGIASDAITASLLKSTVITFAGHQVMLPGA-KGFNRNDHVNLN--PSYFI-FPAWQAFAARTHLTAWRKLQSDGKALLGKMAWGKAQ-----------------LPSDW-----VALRADGRMEPAK-----------------EWPPRM----SYDAI---------------RIPLYLSWADPQS--ALLTPWKSWFQ---SYPRLQTPAWVNVNTNDVAPWFMTG-GLLAVRDLTTGEAQDDPQLSAQDDYYSASLKMLVWLAKNDR

General information: TITO was launched using: RESULT: Template: 5CZL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1487 -15899 -10.69 -55.20 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -10.69 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.256

(partial model without unconserved sides chains): PDB file : Tito_5CZL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5CZL-query.scw PDB file : Tito_Scwrl_5CZL.pdb: