Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCEFISEYSEFQYDFISLAQQRFVSVPEIDLRTMIQNFGDWYFANESSSLNTPSIWLVKWFSWVQNNEKQVAANRKKQEQITSTCQKPQESGYFANLFEEQSESQIVDVTPAKKFPMIEEVGHA 4OU6 Chain:B ((16-69)) ------------ADFIRLAALWGVALREPVTTEELASFIAY-WQA-EGKVFHHVQWQQKLARSLQIGR--------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4OU6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 124 -20554 -165.76 -380.63 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.59 3D Compatibility (PKB) : -165.76 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.646

(partial model without unconserved sides chains): PDB file : Tito_4OU6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OU6-query.scw PDB file : Tito_Scwrl_4OU6.pdb: