Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTSNKDVQACLKVVHDALVDVKAKDILQLDV-SSISNVADAIVIASGTSTRHVKALADNVAEEARKA-----GFRPIGVEGERDAEWILIDLGFVVVHVMLPTARKFYDLESLWRTAPESVA 2GL0 Chain:A ((72-153)) ----NLAIDLCKKIAAGHVFVIYIRNAWPINVLNAIKNVPEVVRIFAATANP-LKVIVAEVEPERRGVVGVVDGHSPLGVETEKDRE------------------------------------

General information: TITO was launched using: RESULT: Template: 2GL0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 162 -6846 -42.26 -90.08 target 2D structure prediction score : 0.34 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -42.26 2D Compatibility (Sec. Struct. Predict.) : 0.34 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.196

(partial model without unconserved sides chains): PDB file : Tito_2GL0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GL0-query.scw PDB file : Tito_Scwrl_2GL0.pdb: