Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQKFKLPLAIAIASAVLVGCSSNKVKEAKPNPLPKLTESNKSLVPVFSRSVSSTNKADPLRLQLDASEGVVFTLDPKGEVAAYRGKQRLWEKKVSKLGLSSGVEAAEGIVVVGNSKGQLFALDQATGEQKWTAQLSGAL-LSPSLVQSG-RVITIANDGTVFAHDVSSGQQVWAYKLPNVQFSLRGQPSPVSLDPRTVLIASANAYVYAIDTISGIPHFQRRVAVSEGRSDIQRLIDIDGDPVVAGQLMVTTSFQGQVTVTDLASQRVVWSEDSSS---T-KRPEVYDNKVFVSSTDGKLTAYDLTTGEQLWQNDSLLNRHLSNPVVLGSDLIVGDLDGVLHLIDPTTGKLIGRSKTSGE-V-NTLRVIEKQLYVSTRKGDLSIWQNR 4IMM Chain:B ((77-353)) -------------------------------------------------------------------------------------------------------IAPLADGMIAASRSGIVSGYMG---SIAWQYNAEDVITGGVGIDDQGSVAVIGTRSGKLIALDARTGAARWVVELASSS-----LAPALISGDK-VIVITNSGTIFGLDINSGATVWQYAT-----------VRGMAKPLALDARTVLIGGADGRIHALDTMTGAPVWTRRVGLLRDIDGTPTVVDHYLYAASYSGQLAGFDMTTGRTMFVSELSS---TKKLTTLADAVIGSSTDGDVVAFNRMTGEKLWENHDLKYRGLTNPVTIGTYIAVGDADGVVHILN--

General information: TITO was launched using: RESULT: Template: 4IMM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1537 -27171 -17.68 -107.40 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -17.68 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.554

(partial model without unconserved sides chains): PDB file : Tito_4IMM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IMM-query.scw PDB file : Tito_Scwrl_4IMM.pdb: