Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHTAVANVKLNWTYFCLIL-----ASTFLQGSSFVATKILLSSMSPLWVATIRFFIAALSLLPLIIYRYFKNPISLFNIPWLKLFVIGLFQTAGVMAFLNIGLGYTSPSTAAILMASNPLLVVIL---------------AMLILG-------ERISIRALVGLIVAFIGVVICIGLGNTNSGGIGRGEVLVILASSCWAIATIINKKFNLHLDPWVITFW--QMLLGSLVLFLVALFSQQPFTLPTTESMWLTFLWLAIPASTGAMG-LWFAALKIGGAIHTSGFLFLCPLFSAIITYFVLGTVLTSQELIGGFLIGTGIYVLSRYR 3G8Q Chain:A ((1-278)) -MKVSLAGQTVDVKKILNEIPKRTVTAALLEGGEIVAVEEADDEH------AERKLVRRHDVEGKVVFVT-ARPC---------LYCARELAEAGVAGVVYLGRG---RGLGPYYLARSGVEVVEVHPDEPLGYDPVDRLDVLLTFGGNPYLTEEDVAARVYCLLTGRGFDADIAPAPENLS----GRVEIMVTRGDPDEAVELLKEELPVFRIRRFLISGEFDRDELRERILEDIEPRILDPFAVRARIAR------------AGAFSSSREAEVFIGDVLTSVGREVNLNDPRTVVTVDVLGPRVSVGVEKR---------------

General information: TITO was launched using: RESULT: Template: 3G8Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1215 -97264 -80.05 -392.19 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -80.05 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.214

(partial model without unconserved sides chains): PDB file : Tito_3G8Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G8Q-query.scw PDB file : Tito_Scwrl_3G8Q.pdb: