Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKPNDVVSNLLNNPTPNTHASVDSAQQEQQAGLDFVRQVITDDLAAGRAKQIVTRFPPEPNGYLHIGHVKAICLNFGVAEEFNGLCNLRFDDTNPDAEEQEYVDGIANDVKWLGFSWNGEPRYASGYFDQLYAWAIQLIEQGDAYVDLQSPEEIKLNRGSFVEPGKNSPYRDASVEENLARFEKMRNGELKEGEAVLRAKIDMASPNVHMRDPILYRVLHSEHHQTGDKWKIYPMYDYAHPLSDAIEGITHSLCTLEFQDHRPFYDWIVEKVKPKAVPHQYESSRLNVDYTITSKRKLRKLVEGGYVNGWDDPRMPTVVGMRRRGFTPEGLRDFCKRVGVSKTDGIVDVAMLEFCIRQSLENTAARGMAVLNPLKVTLTNLPEDLDL-THARHPNV-DMGERVIPLTKEIYIDRKDFEEVPPKGFKRLIPDGEVRLRHAYVIKCDEVIKDANGEVVELKCSIDPDTLGKNPEGRKVKGVIHWVSATKGIPAEVRIYDRLFTEADPETGDDFLANLNPDSLKVVQAVIEPALAQAKPEDRFQFEREGYFVADQYDHTPEKPVFNRVLDLKDSFKPEKK 2RE8 Chain:A ((10-548)) ---------------------------------NFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSDYFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEKMRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCISDALEGITHSLRTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNLLVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIREDLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRADFREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTL----------GVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSLKDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDT------

General information: TITO was launched using: RESULT: Template: 2RE8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2846 -145365 -51.08 -276.36 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.86 3D Compatibility (PKB) : -51.08 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.519

(partial model without unconserved sides chains): PDB file : Tito_2RE8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2RE8-query.scw PDB file : Tito_Scwrl_2RE8.pdb: