Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVQIITIDGPSGSGKGTLAAKLAAYYQFHLLDSGALYRLLGLSLHKHDLLEKLDSHLDECVNYARQLNIKFETSAEGTLVFLDGEDVTQTIRTERVGEYASKVAAIPELRQALFERQRAFAQTPGLVADGRDMATSIFPEANAKIYLTASAESRAERRVKQLQGMGLDAKINDILANIQARDKRDMEREVAPLKPAGDAYIIDSSELTIDQVFKLMVDYVNSRTVSN 3W8N Chain:A ((4-206)) ----IVTIDGPSASGKSSVARRVAAALGVPYLSSGLLYRAAAFLALRAGVDP---GDEEGLLALLEGLGVRLLAQAEGNRVLADGEDLTSFLHTPEVDRVVSAVARLPGVRAWVNRRLKEV--PPPFVAEGRDMGTAVFPEAAHKFYLTASPEVRAWRRA---P-----QAYEEVLRDLLRRDERDKAQSA----PAPDALVLDTGGMTLDEVVAWVLAHI-------

General information: TITO was launched using: RESULT: Template: 3W8N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 922 -123385 -133.82 -616.93 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -133.82 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.624

(partial model without unconserved sides chains): PDB file : Tito_3W8N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3W8N-query.scw PDB file : Tito_Scwrl_3W8N.pdb: