Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MNKL-KLKGRPRKGKFECFSTQAWFYAV-QMV-SNIGTAYALEKVFFPTRFKTVKGKIIRPRLFDRYKMGTITVGKKLVQQVEASYTNTALWFNAVLWKILDERCDDFELNAWFRSLDQNLMQFFFKSTLTVDDSIKIQVKKINQKNFKKLKNY--ELLDVFTLVVLAIQMSIEKKDMDSLDKALYGYHQIRRTLQEHPIFSKFYENLLSTLELHFIQQGEHTFGSPIALMLWEYDPMMSVH-PQMRMATISEGKSVQQTISDASYLVKAQVCCFEKYYCNSDLKATIRLYYSIPLGLSLLPNVDSTVLIPFAKKFFPLARFPEIIAQDKENMIAMTMMFG----DEL----DWESLESMVGIAVLQEIVYFNEDRFVETHTRFWQERLGLIK 5D8M Chain:A ((8-339)) RPVAPLAHAMSPSVLVPAGLAGIQDGRGRFREIMTAIMADHGA--------FLPGDRSSDGDGILWRL----------AGEPGPTGRPVPLGVSTAGIRLVLVPGLLAECVS-ESSLLFDDARPDVERYGYATTL-----VRTGGRWGSARNAAIIHEVVAKLPENDTIVFVTHS---------------KGAVDVLEALVSYPDLAARTAAVVSV--------AGAIDGSPLAETFSDGLLRFAESMP-LSSCPPGEGTEALDSLKRAYRLRFLAE---------HRLPARVR-YYSLAAFASREET--SAILRPFYDILAKTDALNDGLVIAADAIIPGGTLLGYPNADHLAVAMPFSKKPSLLTSVISKN--SYPRPALLEAIARYVEEDL----

General information: TITO was launched using: RESULT: Template: 5D8M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1658 5492 3.31 17.54 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 3.31 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.194

(partial model without unconserved sides chains): PDB file : Tito_5D8M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5D8M-query.scw PDB file : Tito_Scwrl_5D8M.pdb: