Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWQQLITWLKQLSFLVKKEFLTIFSDPANRAILFVPALMQALLSGYAATYDVNHVDYAILDQSNGQISHELISKLDGSGIFKRVATLEYTEQIKQVTDNRQALLIIAIPNDFESKLNNNQSAPIQVIVDGRNSSTAMVAGSYLNKIIGQFNQQKFNSALPISLETRTWYNPNQESRWSLMPALIAALSMMQTLLLSALSVAREREQGTFDQLLVTPYTPLQIMIGKALPPIFVGLMQSTIILLIILFWFKIPMNGSIGLLYFGLFSFNVAVVGVGLSISALSLNMQQAMLFTFLLIMPLMLLSGLLTPVENMPKALQVATYANPLRFGINLVQRVYLEGASFAQVKLNFLPMIVLGIVTLPLAAWLFRNRLS 2MWT Chain:A ((6-29)) -------FFKKVKKSVKKRLKKIFKKPMVIG-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2MWT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 9 -1466 -162.83 -61.06 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.42 3D Compatibility (PKB) : -162.83 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.42 QMean score : 0.786

(partial model without unconserved sides chains): PDB file : Tito_2MWT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MWT-query.scw PDB file : Tito_Scwrl_2MWT.pdb: