Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDLCFDVSNLFRSSLRVIMQHPTSTDIQRVREFLLDLQARICAGLEQQEKAGGGTAEFIIDDWERPEGGGGRSRVLQNGTVIEKGGVMFSHINISKLPPSATERHPQIAGAKAQALGVSLVIHPKNPNIPTSHANVRLFVAEREGQDPIWWFGGGFDLTPFYPDDQDVLNWHQAAYDLCKPFGDNVYAEHKKWCDDYFYLKHGDEQRGVGGLFFDDLNCWDFETCFKYIQAVGNGYLNAILPIFEKHREQPYTEAQREFQLYRRGRYVEYNLVYDRGTLFGLQTGGRIESILVSLPNLAAWSYRPEWDEDSPEKRLTDYYLKPRDWLGLEEKVA 3E8J Chain:B ((7-306)) -----------------------SLPVEAVKEFLLKLQDDICHAIEAEDE----QATFMEDKWTREGGGGGRTRVIANGAVIEKGGVNFSHVYGKG-------------GCNYQAMGVSLVIHPKNPHVPTSHANVRLFVAE--GKEPVWWFGGGFDLTPYYAVEEDCYYFHHVAQELCRPFGADVYERFKAWCDEYFFIPHRNEARGIGGLFFDDLNEWPFEKCFAFMQAVGKGFIDAYIPIVNRRKNTPYTEQQVEFQEFRRGRYAEFNLVIDRGTKFGLQSGGRTESILMSLPPRARWGYNWHPEPGTPEARLTEYFLTKKQWV-------

General information: TITO was launched using: RESULT: Template: 3E8J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1501 -134129 -89.36 -470.63 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : -89.36 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.494

(partial model without unconserved sides chains): PDB file : Tito_3E8J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3E8J-query.scw PDB file : Tito_Scwrl_3E8J.pdb: