Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPIQKCKPTSPGRRFVEKVVHDHLHKGAPYAPLVEAKKRTGGRNNNGHITTRHVGGGHKQHYRIVDFKRNKDGVPAVVERIEYDPNRTAHIALLKYADGERRYIIAPKGLRAGDKVQSGNDAPIRPGNCLPLRNMPIGSTLHNVELKIGKGAQLARSAGASVQLLGRDGSYAIIRLRSGEMRKVHVECRAVIGEVSNQENNLRSLGKAGAARWRGVRPTVRGMAMNPIDHPHGGGEGRNK--GIQPVSPWGQKAKGYKTRTNK-RTTKMIIRDRRVK 3J3V Chain:C ((1-277)) MAIKKYKPTSNGRRGMTTSDFAEITTDKPEKSLLAPLHKKGGRNNQGKLTVRHQGGGHKRQYRVIDFKRDKDGIPGRVATVEYDPNRSANIALINYADGEKRYILAPKGIQVGTEIMSGPEADIKVGNALPLINIPVGTVVHNIELKPGKGGQLVRSAGTSAQVLGKEGKYVLVRLNSGEVRMILSACRASIGQVGNEQHELINIGKAGRSRWKGIRPTVRGSVMNPNDHPHGGGEGRAPIGRKSPMSPWGKPTLGFKTRKKKNKSDKFIVRRRKNK

General information: TITO was launched using: RESULT: Template: 3J3V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1173 -20925 -17.84 -76.37 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain C : 0.86 3D Compatibility (PKB) : -17.84 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.371

(partial model without unconserved sides chains): PDB file : Tito_3J3V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3J3V-query.scw PDB file : Tito_Scwrl_3J3V.pdb: