Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLRNDWTRE---EIQALYEQPFLDLVFKAQQVHREHFTANTIQVSTLLSIKTGKCPEDCKYCSQSAHYDSKLEAEKRIAVEKVISEAKAAKDSGSSRFCMGAAWRNPHERDMPYVL-EMVREVKALGMETCMTLGMLNQSQAERLKDAGLDYYNHNLDTSRKYYSHIISTRT-FDDRLNTLDYVRQAGMKVCSGGIVGL-GESREDRIGLL-----HELATLPIHPESVPINMLVPIEGTPLADVEKLDVIEWIRTIAVARIIMPHSYIRLSAGRESLSDSDQALAFMAGANSLFSGDKLLTTPNAGEGKDQALFNKLGLTAEKPKPTVSDLSVDAMSA 5FF4 Chain:A ((22-300)) ---RREFTREVLKEALSINDRGFNEALFKLADEIRRKYVGDEVHIRAIIEF-SNVCRKNCLYCG--LRRDNKNLKRYRMTPEEIVERARLAVQFGAKTIVLQSG-EDPYY--MPDVISDIVKEIKKMGVAVTLSLGEWPREYYEKWKEAGADRYLLRHETANPVLHRKLRPDTSFENRLNCLLTLKELGYETGAGSMVGLPGQTIDDLVDDLLFLKEHDFDMVGIGP-------FIPHPDTPLANEKKGDFTLTLKMVALTRILLPDSNIPATTAMGTIVPGGREITLRCGANVI---------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FF4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1344 13693 10.19 51.09 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : 10.19 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.482

(partial model without unconserved sides chains): PDB file : Tito_5FF4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FF4-query.scw PDB file : Tito_Scwrl_5FF4.pdb: