Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------MTKKNNA-----PELHKYRGLTSTEQM-VIHQMLISYVR-EENCRFNIIMTGTAEP------Y-----NLVKLTS---------INFENEASA-----IWVHFETITGEQIALPIDFLSRIEFSGQQEI 4JDN Chain:A ((6-149)) KAFNNFPITNKIQCNGLFTPSNIETLLGGTEIGKFTVTPKSSGSMFLVSADIIASRMEGGVVLALVREGDSKPCAISYGYSSGVPNLCSLRTSITNTGLTPTTYSLRVGGLESGVVWVNALS-NGNDI-LGITNTSNVSFLEVIPQ

General information: TITO was launched using: RESULT: Template: 4JDN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 321 9323 29.04 98.13 target 2D structure prediction score : 0.34 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 29.04 2D Compatibility (Sec. Struct. Predict.) : 0.34 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.268

(partial model without unconserved sides chains): PDB file : Tito_4JDN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JDN-query.scw PDB file : Tito_Scwrl_4JDN.pdb: