Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKITALLFALCATVTHAELQTLDNDALQAINGQAGADLSLMLNLNQTSNGIFDNGSGGVCENVEFCHIGLAINKRFVQADTTKPSGWAENSDSGNKLWLVFKGVQGTLNIQKMGLDGADLKYLDKSNTEIIKLAIQLSFSAAQPILIRNFGFNALSIEQDNFTSSTTTQGSSANMYDYAYFKKPTYAATANSANQQVATATPSAYDYGRETGFMGLMMNGNMAIQGKVMVFSCDGSHPRC 3N27 Chain:B ((42-56)) -----------------------------YSGGSGGIDISIELN-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3N27.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 12 -133 -11.08 -8.87 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain B : 0.52 3D Compatibility (PKB) : -11.08 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.786

(partial model without unconserved sides chains): PDB file : Tito_3N27.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N27-query.scw PDB file : Tito_Scwrl_3N27.pdb: