Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPFFGFTPSEGLLNDIQTGIAN-KNSS-EPLYPLRDKIALQLNEEIIENVLT---QLVQ---------HFPASEK-----RDTAEKLAGYVKSTVAVLLKQLLSKAPNDVVKQSVEFSEKSLFKDP-QGQYKVGVALDAGLVTNLKHNFAELQAGNDINKAALAELYKQFGDAM-VRHFMSDFNKTL-DLGMIKRKAADIGAAAVTKAVHIAIDKLIPSLNR-TELKAMAEY-HDGLFFN---- 4G3A Chain:A ((5-231)) KP-----------SDLDGFIQQMPKADMRVKVQLAEDLVTFLSDDTNSIVCTDMGFLIDGLMPWLTGSHFKIAQKSLEAFSELIKRLGSDFNAYTATVLPHVIDRLGDS--RDTVREKAQLLLRDLMEHRVLPPQALIDKLATSC-FKHKNAKVREEFLQTIVNALHEYGTQQLSVRVYIPPVCALLGDPTVNVREAAIQTLVEIYKHVG---DRLRPDLRRMDDVPASKLAMLEQKFDQVKLE

General information: TITO was launched using: RESULT: Template: 4G3A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 846 24163 28.56 121.42 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 28.56 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.206

(partial model without unconserved sides chains): PDB file : Tito_4G3A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4G3A-query.scw PDB file : Tito_Scwrl_4G3A.pdb: