Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGANSTLIFGILINIAIL-LVFFRFLMQLAAVSPYNPVVLSTVKATKIVDIFGRIFPTVAKGR-FNL-AALVLLIILYLLKIFGVMYLSGSMPNSPVHLVILTFVTMIQDLIRFCRYLIFATIILSWVVMFTQSRSPYIEVIQDLAEPLLAPFRHLLPNMGMIDLSPILAFLALYIAEILMNEVAKVLLTGL 3PMI Chain:A ((4-125)) -------------SFEVGMLVWHKH-----KKYPFWPAVVKSVRQRD-----KKASVLYIEGHMNPKMKGFTVS--LKSLKHFDCKEKQTLLNQAREDF---------NQDIGWCVSLITDYRVRLGCGSFAGSFLEYYA--ADISYPVRKSIQQDVL----------------------------------

General information: TITO was launched using: RESULT: Template: 3PMI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 465 -39069 -84.02 -328.31 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.49 3D Compatibility (PKB) : -84.02 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.49 QMean score : 0.199

(partial model without unconserved sides chains): PDB file : Tito_3PMI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PMI-query.scw PDB file : Tito_Scwrl_3PMI.pdb: