Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MIT----ANKGEWSEVYA--------LFKLLSEG-KLYAGDKDLNKIPNLIYPI--------ISILRQESQDLLTYSPEPNHGVVHIQNGTSTFT----ISQSDFKGITELLLTE-IKKKQPTASFSIPEVETFISQYNSKKIKAKSSAKSDIRIIIYDQKIGTTPELGFSIKSKLGKASTLLNASQATNFVYKVKNLTLSPQEIDDFNQLEFTVPIIQGRIHHLESLGG-SVEFSHITNDVFNNNLILLDSLLPNIISEALYKYYTSPITSVKEIFEQVASANPIHY----SLQHQHPFYEYKVKKLLCEIAIGMMPSTVWTGNNIDATGGYLVIKEDGDV--ICYHLYHRHEFEEYLYNNTRFEAASKSRHNYGNLFIENGELYMTLNLQIRFK-- 4K7J Chain:A ((26-375)) GLLTDYGNASASPWMKKLQSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDGGIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNTGDFPLGGILAHTGSGGSMTLTASDGIASKQRVSLFAKPLLAEQTLTVNGNTVSANGGGW---------QVLDTGAALPLTIHTEMPWDIGFINIENPAGGIT-VSAMGINGAQLTQWSKWR------ADRMNDLAQTGADLVILSYGTNEAFNNNI-------------DIADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLG---------VCGTRPVRLTEVQQMQRRVARQGQTMFWSWQNAMGGICS-----------MKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRSA

General information: TITO was launched using: RESULT: Template: 4K7J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1838 57762 31.43 183.96 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 31.43 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.058

(partial model without unconserved sides chains): PDB file : Tito_4K7J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4K7J-query.scw PDB file : Tito_Scwrl_4K7J.pdb: