Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNQEIVVILGNADKRDWHFILVQLFFQLLGRRDRLFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFSSLGIFTFFYYQNIPMKYL----LKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSRFVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVI--TKLLDS----RPEQTIEKATLKERFHEEPLVIIDSVFYSA------ALFLSVYVSHGLNLHNPYWLTLSCASILLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARNYAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVI-GSFIGLGVAWVMT------VGLQHYLTVVRQKLE----------- 5T6O Chain:A ((1-389)) ----SAFEVGRNVAVTEGAVVFENEYFQLLQYKPLT-------DKVHARPLLMVPPCINKYYILDLQPESS--LVRHVVEQGHTVFLVSWRNPDASMAGSTWDDYIEHAAIRAIEVARDISGQDKINVLGFAVGGTIVSTALAVLAARGEHP-AASVTLLTTL-----LDFADTGILDVFVDEGHVQGAPCALLRGLELANTFSFLRPNDLVWNYVVDNYLKGNTPVPFDLLFWNGDATNLPGPWYCWYLRHT---YLQNELKVPGKLTVCGVPVDLASIDVPTYIYGSRED-------HIVPWTAAYASTAL--LANKLRFVLGASGHIAGVINPPAKNKRSHWTNDALPESPQQWLAGAIEHHGSWWPDWTAWLAGQAGAKRAAPANYGNARYRAIEPAPGRYVKAKA

General information: TITO was launched using: RESULT: Template: 5T6O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1946 -239857 -123.26 -695.24 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -123.26 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.149

(partial model without unconserved sides chains): PDB file : Tito_5T6O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5T6O-query.scw PDB file : Tito_Scwrl_5T6O.pdb: