Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEKKMKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGVAAGVVQSFQNCLRENN----ISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFLAKRQTR---------LND-------IDLGNKKK-IEIVNAF--LP--VKHLNVDRVSETISSLERERAEVLVSSMAFGVDNGEPERVVYEAASVK------S-IPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTEDSVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYL---RASNGVYFEVGGTTTNIGVIKNGRPAI--DYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSG-IIDVGPRSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANVLGLVQEEHFSYG----NVPSARKAI-QALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKYHLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERIIPSPS---------------KEDIRSLKNEAMNKAIESGATPESIEVHVEIDP----QTSKVTAIATGSTEVKATDLTKEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKVQRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQLQLLMDLEVSTMEPEEEVIVVAGNIKQT 5L9W Chain:B ((8-581)) ------DAINSIDIDVGGTFTDFVLTLD--GE-RHIAKCPTTPHD---LSIGFLNAVEAGGDKVGLSVEELLPRIDIIRYSTTVALNRLLQRQGPRIGLLTTEG-HEDAILIGRGAQWTDGQRVAERRNIAVQNKPLPLIERDLILGVRERIDSSGSVVRPLDEEDVRTKLRMLMDRGARAIVVSLLWSFMNPAHEKRVREIIREEYKEYHIGFVPVVMSHSVVSKIGEYERTMTAVLDAYLQRSMQNDIGATWDKLRAKGYHGAFLMIHNSGGSAD--IFKTPASRTFNGGPVAGLMGSAYFANKLGYKNVVAGDVGGTSFDVALVVESSVRNYTFRPVIDKWMVNVTMMQTISVGSGGGSIAKVDRSGTRLEVGPRSAG---------SMP---------------G-PVCY----DLGGT--EPTVTDADVVLGYINPDTYYGGRMPLNKAKAEKAIREKIAQPLGIETIEAAALIRYIVDENMASAIKREVHMRGYHPEDFVLFAFGGAGPTHMAGLKGDIP-KAVVFPAAPVFCAMGSSIMDIVHMYEQSRRMVFMEPGTEKFVVDYEHFNQTVDTMIERARQELRSEGLEVDDASFGLELDMLYGGQVNLKRMSS-----------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5L9W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2883 25083 8.70 48.99 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : 8.70 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.446

(partial model without unconserved sides chains): PDB file : Tito_5L9W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5L9W-query.scw PDB file : Tito_Scwrl_5L9W.pdb: