Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFRRYPYSDSSNSEEKRRKNMKYFQLVLTLITAMVIEVLEQKLA 1SPF Chain:A ((1-35)) --LRIPCCP------VNLKRLLVVVVVVVLVVVVIVGALLMGL-

General information: TITO was launched using: RESULT: Template: 1SPF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 12 -965 -80.42 -27.57 target 2D structure prediction score : 0.89 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -80.42 2D Compatibility (Sec. Struct. Predict.) : 0.89 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.298

(partial model without unconserved sides chains): PDB file : Tito_1SPF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SPF-query.scw PDB file : Tito_Scwrl_1SPF.pdb: