Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIKIGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSEALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY-D-----NLALYEYVIDLCLSRGGTLF 2CZV Chain:A ((31-163)) VVSIKFNEEV-------------DKEKLREARKEYGKV---AILLSNPK--PSLVRDTVQKFKSY--LIYVESN-------------D--------LRVIRYSIEK-GVDAIISPWVNRKDP--------GID---HVLAKLMVKKNVALGFSLRPLLYSNPYERANLLRFMMKAWKLVEKYKVRR-

General information: TITO was launched using: RESULT: Template: 2CZV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 532 -53965 -101.44 -424.92 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -101.44 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.367

(partial model without unconserved sides chains): PDB file : Tito_2CZV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CZV-query.scw PDB file : Tito_Scwrl_2CZV.pdb: