Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWIAHKMDMSMKLIHQAERYLAEKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNE---YQKEALVYEDIGEDTIDVYLDKK--IIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLVKEMRKRQEERRVNDENSK 2DO3 Chain:A ((466-520)) ---------------------------------------------QELRKYFKMGDHVKVIAGRFEGDTGLIVRV-EENFVILFSDLTMHELKVLPRDLQL------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2DO3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 166 -614 -3.70 -12.27 target 2D structure prediction score : 0.24 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -3.70 2D Compatibility (Sec. Struct. Predict.) : 0.24 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.591

(partial model without unconserved sides chains): PDB file : Tito_2DO3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2DO3-query.scw PDB file : Tito_Scwrl_2DO3.pdb: